Mouse Perineurial Fibroblasts (MPNF)

Product Description

Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic conditions, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles [1]. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100 [3].

iXCells Biotechnologies provides high quality Mouse Perineurial Fibroblasts (MPNF), which are isolated from postnatal day 8 C57BL/6 mouse sciatic nerve and cryopreserved at P1, with >0.5 million cells in each vial. MPNF express vimentin, S-100, GFAP and CD90. MPNF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies. 

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References

[1] Salzer JL. (1999) Creating barriers: a new role for Schwann cells and desert hedgehog. Neuron. 22: 627-9. 
[2] Erlandson RA. (1991) The enigmatic perineurial cell and its participation in tumors and in tumor like entities. Ultrastruct Pathol. 15: 335-51. 
[3] Ariza A, Bilbao JM, Rosai J. (1988) Immunohistochemical detection of epithelial membrane antigen in normal and perineurial cells and perineurioma. Am J Surg Pathol. 12: 678-83.