Adipocyte Basal Medium

Recombinant Human FGF2

产品代码: IX03116
Call for Price: 0592-7821662

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1 mg
10 ug

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Product Description

Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

Product Details

  Alternative Names

  bFGF, Fibroblast growth factor 2, FGF-2, Heparin-binding growth factor 2, HBGF-2
  Recombinant Human Basic Fibroblast Growth Factor (FGF2)

  Gene Symbol

  FGF2

  Source

  E. coli

  Appearance

  Lyophilized Powder

  Molecular Weight

  17.0 kDa

  Purity

  >95% as determined by SDS-PAGE

  Endotoxin Level

 

  Biological Activity

  The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.

  Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS

  Reconstitution

  A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL is recommended. Please note, filter sterilization is a must following reconstitution. This solution can then be diluted into other buffers.

  Storage

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

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