Human Adipose Derived Stem Cells (ADSCs, Obesity)

Product Description

Human Adipose-Derived Stem cells (ADSCs) are isolated from human lipoaspirate tissue collected during elective surgical liposuction procedures. ADSCs are available for normal donors, patients with Type 1 Diabetes, Type 2 Diabetes, or Obesity (BMI>30). It has been shown that the ADSCs demonstrate very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells. Thousands of articles have been published on ADSCs using a variety of terminology, including preadipocytes, adipose-derived mesenchymal stem cells (AD-MSCs), adipose MSCs (AMSCs), adipose-derived adult stem (ADAS) cells, and adipose stromal/stem cells (ASCs). Normal human ADSCs have been reported to differentiate into many different lineages including chondrogenic, osteogenic, adipogenic and neural. And have been applied in studies include stem cell differentiation, regenerative medicine ^[1], and cell therapy ^[2].

iXCells Biotechnologies offers normal human adipose-derived stem cells (hADSC) from adipose tissues from single donor, and cryopreserved at passage 1. hADSC are positive for CD29, CD44, CD73, CD90, CD105, and negative for CD14, CD31, CD45 (Figure 1). hADSC can be in vitro differentiated into adipocytes and osteoblasts (Figure 2 and 3) using Adipocyte Differentiation Medium (Cat # MD-0005) and Osteogenic Differentiation Medium (Cat # MD-0006), separately. hADSC can be further expanded for 3-4 passages using Adipose-Derived Stem Cell Growth Medium (Cat# MD-0003). These cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

Figure 1.  Immunostaining of cell surface markers of human ADSC.

Figure 2. Human ADSC adipocyte differentiation (Day 14 post adipogenic induction).

Figure 3. Human ADSC osteogenic induction (Day 24 post osteogenic induction). Alizarin Red S staining of osteoblasts. The extracellular calcium deposit was stained in bright orange-red color.

Product Details

References

[1]  Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.

[2]  Bertheuil N, et al and Tarte K. Adipose-derived stromal cells: history, isolation, immunomodulatory properties and clinical perspective. Ann Chir Plast Esthet. 2015;60(2): 94-102.

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 Adipocyte Differentiation Protocol

 

 Osteogenic Differentiation Protocol