Human Peripheral Blood CD14+ Monocytes (Positive Selection)

Product Description

Monocytes are a type of white blood cells (leukocytes). Monocytes constitute 10~30% of peripheral blood mononuclear cells in the human body. They play multiple roles in immune function including replenishing resident macrophages under normal states. And in response to inflammation signals, monocytes can move quickly (approx. 8–12 hours) to sites of infection in the tissues and divide/differentiate into macrophages and dendritic cells to elicit an immune response ^[1]. CD14 is an important surface marker for monocytes.

iXCells Biotechnologies offers CD14+ monocytes isolated from normal human peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic selection.  > 90% of the cells are CD14+ as showed by flow cytometric analysis (Figure 1 A). And >53% of monocytes are capable to differentiate into mature dendritic cell (Fig 1B, C).

Figure 1. Flow cytometric analysis showed A. >90% of purified cells are CD14 positive. B. C. Flow cytometric analysis of monocytes dendritic cell differentiation, showed >53% of cells are CD83 positive and >77% cell are CD1a positive.

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References

[1]  Ziegler-Heitbrock, L (2007). "The CD14+ CD16+ Blood Monocytes: their Role in Infection and Inflammation, Review". J Leukocyte Biology 81 (3): 584–92.

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