Human Peripheral Blood CD19+ B Cells (Positive Selection)

Product Description

B cells, also known as B lymphocytes, are a subtype of lymphocyte in white blood cells ^[1]. They play a critical role in the humoral immunity component of the adaptive immune system by secreting antibodies^[1]. They also function in immune system as antigen presentation cells and by secretion of cytokines^[1].

CD19 (Cluster of Differentiation 19) is an important surface marker for B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 ^[2] and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase.

iXCells Biotechnologies offers CD19+ B Cells isolated from normal human peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic selection. > 90% of the cells are CD19+ as showed by flow cytometric analysis (Figure 1).

Figure 1. Flow cytometric analysis showed that >90% cells are CD19 positive. 

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References 

[1]  Murphy, Kenneth. 2012. Janeway’s Immunobiology 8^th Edition. New York, NY: Garland Science.

[2]  Bradbury LE, Kansas GS, Levy S, Evans RL, and Tedder TF. “The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules”. Journal of Immunology 1992; 149 (9):2841-2850.

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