Human Renal Glomerular Endothelial Cells (HRGEC)

Product Description

Human primary renal glomerular endothelial cells (HRGEC) are a specialized microvascular cell type involved in the regulation of glomerular ultrafiltration. They form the inner part of the filtration barrier and are involved in pathophysiological processes in the glomerulum [1]. HRGEC constitutively produce bio-active molecules, which can be amplified by inflammatory and thrombotic molecules [2]. Endothelial cell injury due to a severe glomerular lesion can inhibit angiogenesis and result in sclerosis at the injured site [3,4]. HRGEC injury affects mesangial and epithelial cells and leads to the progression of renal disease [4]. The biological properties of HRGEC remain largely unknown because of difficulties associated with the culturing, cloning and propagation of these cells.

iXCells Biotechnologies provides high quality HRGEC, which are isolated from human kidneys and cryopreserved at P0 after purification, with >0.5 million cells in each vial. HRGEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM), and by the formation of microtubular structures in vitro. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRGEC can proliferate in Endothelial Cell Growth Medium (Cat# MD-0010), but they are not recommended for further expansion, because the purity of the endothelial population may decrease. 

Product Details

References

[1] Nangaku, M., Shankland, S. J., Couser, W. G. and Johnson, R. J. (1998) A new model of renal microvascular injury. Curr Opin Nephrol Hypertens 7(4):457-62. 
[2] Kester, M., Nowinski, R. J., Holthofer, H., Marsden, P. A. and Dunn, M. J. (1994) Characterization of platelet-activating factor synthesis in glomerular endothelial cell lines. Kidney Int 46(5):1404-12.
[3] Lee, L. K., Meyer, T. W., Pollock, A. S. and Lovett, D. H. (1995) Endothelial cell injury initiates glomerular sclerosis in the rat remnant kidney. J Clin Invest 96(2):953-64.
[4] Yamanaka, N. and Shimizu, A. (1999) Role of glomerular endothelial damage in progressive renal disease. Kidney Blood Press Res 22(1-2):13-20.

 Download Datasheet