Human Skeletal Muscle Cells (HSkMC)

Product Description

Skeletal muscle cells, one of the largest cell types in the body, are multinucleated cells formed by the fusion of myoblasts. Skeletal muscle regeneration is a complex process. When skeletal muscle is injured, quiescent resident myoblasts called satellite cells are activated to proliferate, migrate, and differentiate [1]. Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase (MAPK) have been suggested to play an important role in skeletal muscle growth [2]. Insulin-stimulated glucose transport in cultured human skeletal muscle cells is mediated by GLUT4 and heparan sulfate proteoglycan is involved in skeletal muscle differentiation [3]. The fusion of mononucleated cells to form multinucleated myotubes is a central event in skeletal muscle development. Controlling the onset and progression of this process is a complex set of interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful model for studying the process of cell differentiation.

iXCells Biotechnologies provides high quality Human Skeletal Muscle Cells (HSkMC), which are isolated from human trapezius muscle and erector spinae muscles of the back and cryopreserved at P0, with >0.5 million cells in each vial. HSkMC express myosin, actin and actinin. These HSkMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung and can further expand for 13 population doublings in Skeletal Muscle Cell Growth Medium (Cat# MD-0052) under the condition suggested by iXCells Biotechnologies.

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References

[1] Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78. 
[2] Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404.
[3] Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V. D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8.