Human Skeletal Muscle Satellite Cells (HSkMSC)

Product Description

Skeletal muscle contains both differentiated myofibers and stem cells, known as satellite cells. The satellite cells, comprising around 1% of the total muscle nuclei, are situated between the plasma membrane of the multinucleated muscle cells and the basal lamina that surrounds each myofiber. In adult muscle, satellite cells are quiescent but proliferate in response to muscle injury, producing myoblasts that can either form new satellite cells or fuse with one another or pre-existing multinucleated muscle cells to help repair the muscle. They are responsible for postnatal muscle growth, hypertrophy and regeneration of skeletal muscle [1]. When quiescent satellite cells are activated, they co-express the transcription factors Pax7 and myoD [2]. 

iXCells Biotechnologies provides high quality Human Skeletal Muscle Satellite Cells (HSkMSC), which are isolated from human muscle of the pectoral girdle and cryopreserved at P1, with >0.5 million cells in each vial. HSkMSC express myosin, actin and actinin. These HSkMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 12 population doublings in Skeletal Muscle Cell Growth Medium (Cat# MD-0052) under the condition suggested by iXCells Biotechnologies.

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References

[1] Villena, J., Brandan, E. (2004) Dermatan sulfate exerts an enhanced growth factor response on skeletal muscle satellite cell proliferation and migration. J Cell Physiol. 198(2):169-78. 
[2] Morris, R. T., Spangenburg, E. E., Booth, F. W. (2004) Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol. 96(1):398-404. 
[3] Al-Khalili, L., Chibalin, A. V., Kannisto, K., Zhang, B. B., Permert, J., Holman, G. D., Ehrenborg, E., Ding, V. D., Zierath, J. R., Krook, A. (2004) Insulin action in cultured human skeletal muscle cells during differentiation: assessment of cell surface GLUT4 and GLUT1 content. Cell Mol Life Sci. 60(5):991-8.

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