Mouse Preadipocytes - brown fat (MPAd-bf)

Product Description

Adipose tissue is critically involved in energy storage and metabolic homeostasis of the body. Adipocyte dysfunction is involved in the pathogenesis of obesity-related insulin resistance. Adipocytes are also immunologically active and they play a role in innate immunity as well as adaptive immunity. Preadipocytes are self-renewing progenitors of mature differentiated adipocytes and can be found in adipose tissue throughout life. Previously, the majority of adipocyte research was conducted in mouse 3T3-L1 preadipocyte lines. 

iXCells Biotechnologies provides primary mouse preadipocytes (MPAd) isolated from mouse interscapular brown fat tissue, which can be chemically differentiated to adipocytes in vitro (>90% efficiency). MPAd can be used as primary cell models to study adipocyte function and related metabolism and obesity research. These fibroblast-like adipocyte precursor cells can be propagated two passages in Preadipocye Growth Medium (Cat # MD-0004) prior to differentiating into mouse adipocytes (MAd).  iXCells provides optimized protocol for adipocyte differentiation.  Mature MAd are expected 7~14 days after initiation of differentiation and should remain healthy and responsive for at least 1 week after complete differentiation. MAd are ideal mouse cellular models for drug discovery research in the area of obesity, diabetics and cardiovascular diseases. 

Figure 1. Mouse preadipocytes (A) and differentiated adipocytes (B and C). Mouse adipocytes (B) were differentiated 5 days from primary mouse preadipocytes (A) and cells were stained with hematoxylin and Oil-O-Red, showing lipid droplet formation in differentiated mouse adipocytes. C shows phase contrast image of MAd.

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References

[1]  Arianna Maiorana, Chiara Del Bianco and Stefano Cianfarani. Rev Diabet Stud (2007) 4:134-146. Adipose Tissue: A Metabolic Regulator. 
[2]  Yao, Y., M. Suraokar, B.G. Darnay, B.G. Hollier, T.E. Shaiken, T. Asano, C.-H. Chen, B.H.-J. Chang, Y. Lu, and G.B. Mills. Sci. Signal. (2013) 6(257): p. ra2. BSTA Promotes mTORC2-Mediated Phosphorylation of Akt1 to Suppress Expression of FoxC2 and Stimulate Adipocyte Differentiation. Science signaling. 
[3]  Zhang, L.J., Guerrero-Juarez, C. F., Hata, T., Bapat S.P., Ramos, R., Plikus, M.V., Gallo, R.L. Science (2015) 347(6217):67-71, Dermal adipocytes protect against invasive Staphylococcus aureus skin infection.
[4]  Olivia Osborn & Jerrold M Olefsky. Nature Medicine (2012), 18 (3): 363-374, The cellular and signaling networks linking the immune system and metabolism in disease.

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