Human Preadipocytes / Adipocytes (hPAds)

Product Description

Normal Human  Preadipocytes (hPAds) are isolated from normal human lipoaspirate tissue collected during elective surgical liposuction procedures or dermal adipose tissues. It has been shown that the hPAds demonstrate very similar phenotypic and functional characteristics to that of bone marrow-derived mesenchymal stem cells. Thousands of articles have been published on hPAds using a variety of terminology, including preadipocytes, adipose-derived mesenchymal stem cells (AD-MSCs), adipose MSCs (AMSCs), adipose-derived adult stem (ADAS) cells, and adipose stromal/stem cells (ASCs). Normal human hPAds have been reported to differentiate into many different lineage including chondrogenic, osteogenic, adipogenic and neural. And have been applied in studies include stem cell differentiation, regenerative medicine ^[1], and cell therapy ^[2].

Each lot of iXCells normal human hPAds originates from a single donor of human lipoaspirate tissue. These cells are expanded for one passage inPreadipocyte Growth Medium (Cat# MD-004) and then cryopreserved at primary passage. iXCells preadipocytes are positive for CD29, CD44, CD73, CD90, CD105, and negative for CD14, CD31, and CD45 (Figure 1). Pertinent additional donor information, including Body Mass Index (BMI) may be available upon request. All the cells provided by iXCells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C.  A Certificate of Analysis is provided for each cell lot purchased.

Diseased state ADSCs from donors with Type 1 Diabetes (T1D), Type 2 Diabetes (T2D), Alzheimers's Disease (AD), Parkinson's Disease (PD), Amyotrophic Lateral Sclerosis (ALS) are available as separate products. 

Figure 1.  Immunostaining of cell surface markers of human preadipocytes.

Product Details

References

[1]  Harasymiak-Krzyzanowska I et al. Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 2013; 18(4): 479-493.

[2]  Bertheuil N, et al and Tarte K. Adipose-derived stromal cells: history, isolation, immunomodulatory properties and clinical perspective. Ann Chir Plast Esthet. 2015;60(2): 94-102.

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