Human Bronchial Epithelial Cells (HBEpC)

Product Description

The respiratory epithelium is composed of a mixed population of ciliated, nonciliated, and mucous-secreting cells from proximal to distal airways. The individual characteristics of these cells create not only an effective physical barrier against various noxious substances, but also a highly sophisticated host defense system by producing and releasing a large number of chemical mediators and cytokines [1]. The bronchial epithelium consists of the surface epithelial cells and mucus glands. The surface epithelial cells are made up of three principle cell types: basal, goblet, and ciliated cells, of which the latter two form a suprabasal columnar structure and are necessary for mucociliary clearance. Studies using human primary bronchial epithelial cells (HBEpC) have demonstrated that IL-4 and IL-13 stimulation can modify cellular proliferation, ciliary beating, and mucous production [2]. HBEpC proliferation is also regulated in part by EGF receptor signaling [3]. HBEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. 

Figure 1. Human Bronchial Epithelial Cells (HBEpC) (phase contrast).

iXCells Biotechnologies provides high quality HBEpC, which are isolated from human bronchi and cryopreserved at P1, with >0.5 million cells in each vial. HBEpC express cytokeratin-18, -19, and vimentin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Epithelial Cell Growth Medium (Cat# MD-0041) under the condition suggested by iXCells Biotechnologies.

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References

[1]. Velden, V. H., and H. F. Versnel. 1998. Bronchial epithelium: morphology, function and pathophysiology in asthma. Eur. Cytokine Netw. 9:585-597. 
[2]. Kikuchi, T., Shively, J.D., Foley, J.S., Drazen, J.M., Tschumperlin, D.J. (2004) Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation. Am J Physiol Lung Cell Mol Physiol. 287:L119-26. 
[3]. Kim S, Schein AJ, Nadel JA.(2005) E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 289:L1049-60.

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